What are the major branches of life science?

What are the major branches of life science?
What are the major branches of life science?
What are the major branches of life science?

What are the major branches of life science? Life Sciences elucidated

What are the major branches of life science ? Do you have interest  in our research? Do you want to know more or you want to fund our reasearch? contact us here.

Life Sciences elucidated

Life Sciences elucidated

What are the major branches of life science?: what We do.

Infection and immunology

  • Signaling study; RNA isolation and quantification using qPCR.

  • Protein extraction and quantification using western blotting.

  • Immunoflourescence and protein localisation using confocal laser scanning microscopy.

  • Usage of software such as qPCR analysing programme, Biorad microplate reader , ZEISS examiner, IMAGEJ image analyser, Odyssey infrared imaging system, Kodak imaging system etc.

  • Bacterial cultivation and infection; multiplicity of infection (MOI).

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  • Cell culturing.

  • Generation of polyplexes by self assembly method.

  • Development and optimisation of a robust procedure for localisation of nanoparticles in the cell.

  • Protein isolation, identification and quantification.

  • Identification of peptide and nucleic acids using the bioinformatic tools.

  • Isolation and identification of protein biomarker in cell sample by immunolabelling.

  • Endocytosis pathways in live and fixed cells.

  • Mechanism of delivery of molecules in cells.

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  • Development of sensor based on RNA aptamer.

  • Establishment of protocols for gene therapy.

  • Establishment of protocols for analysis of cellular endocytic vesicles.

  • confocal microscopy  and  immunofluorescence.

  • Colocolisation of nanoplexes with caveolae,  ascertaining the relationship and their location in the caveolae.

  • Confocal microscopy image acquisition and analysis.

  • Transfection with luciferace plasmid.

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Int J Biol Macromol. 2012 Dec;51(5):1043-51. doi: 10.1016/j.ijbiomac.2012.08.016. Epub 2012 Aug 27.

Cellular uptake of DNA-chitosan nanoparticles: the role of clathrin- and caveolae-mediated pathways.Garaiova Z1, Strand SP, Reitan NK, Lélu S, Størset SØ, Berg K, Malmo J, Folasire O, Bjørkøy A, Davies Cde L.

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The success of gene therapy depends on efficient delivery of DNA and requires a vector. A promising non-viral vector is chitosan. We tailored chitosan to optimize it for transfection by synthesizing self-branched and trisaccharide-substituted chitosan oligomers (SBTCO), which show superior transfection efficacy compared with linear chitosan (LCO). The aim of the work was to compare the cellular uptake and endocytic pathways of polyplexes formed by LCO and SBTCO. Both polyplexes were taken up by the majority of the cells, but the uptake of LCO was lower than SBTCO polyplexes. LCO polyplexes were internalized through both clathrin-dependent and clathrin-independent pathways, whereas SBTCO polyplexes were primarily taken up by clathrin-independent endocytosis. The different level of cellular uptake and the distinct endocytic pathways, may explain the difference in transfection efficacy. This was supported by the observation that photochemical internalization increased the transfection by LCO polyplexes considerably, whereas no effect on transfection was found for SBTCO polyplexes.

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Davies C deL, Garaiova Z, Reitan NK, Bjørkøy A, Folasire O, Størset S, Berg K, Strand SP:

DNA-chitosan nanoparticles in gene delivery: Endocytotic pathways and intracellular trafficking.

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